Advertisements
Advertisements
प्रश्न
What is PCR? Explain different steps involved in it.
उत्तर
Definition: Polymerase Chain Reaction (PCR) is the process of in vitro amplification of gene of interest using a PCR machine.
Mechanism of PCR: At the start of PCR, all the requirements are mixed together in ‘eppendorf tube’ and the following operations are performed sequentially:
Step i: Denaturation
The reaction mixture is heated to a temperature (90–98o C) to separate two strands of desired DNA. This is called denaturation.
Step ii: Annealing
The mixture is allowed to cool (40–60o C) that permits pairing of the primer to the complementary sequences in DNA. This step is called annealing.
Step iii: Primer extension / Polymerization
The temperature (70–75o C) allows thermostable Taq DNA polymerase to use single-stranded DNA as template and adds nucleotides. This is called primer extension. It takes around two minutes duration.
संबंधित प्रश्न
Name and explain the technique used for separating DNA fragments and making them available for biotechnology experiments.
Expand the following abbreviation: BAC
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
ECoRI is obtained from ______.
The vector Baculovirus is used for gene transfer in ____________.
Which molecules are called molecular scissors? Why are they called so?
The rDNA technology has provided a method to control the nematode parasite Meloidogyne incognita. Explain the principle involved in this technique.
Nucleic acid segment tagged with a radioactive molecule is called ______.
DNA fingerprinting refers to ______.
What is polymerase chain reaction (PCR)?
