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प्रश्न
Name and explain the technique used for separating DNA fragments and making them available for biotechnology experiments.
Name and describe the technique that helps in the separation and isolation of DNA fragments.
उत्तर
Gel electrophoresis is a technique which is used for separating DNA fragments in a gel on the basis of their electric charge and size.
The process of gel electrophoresis is as follows:
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The fragments obtained after cutting with restriction enzymes are separated by using gel electrophoresis.
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Electric field is applied to the agarose gel matrix and the negatively-charged DNA fragments move towards the anode.
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Fragments separate according to their size by the sieving properties of agarose gel. The smaller the fragment, the farther it moves.
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Staining dyes such as ethidium bromide, upon exposure to UV radiation, are used to visualise the DNA fragments.
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DNA fragments are visible as bright orange-coloured bands in the agarose matrix.
- These bands are cut from the agarose gel and extracted from the gel piece (elution).
- DNA fragments are purified and used in constructing recombinant DNAs
संबंधित प्रश्न
State the role of DNA ligase in biotechnology.
Give an example of the source of thermostable enzyme DNA polymerase.
The biological scissor is ______.
Write a short note on Electrophoresis.
In vitro amplification of DNA or RNA segment is known as ___________.
What is Recognition sequence? Explain in brief.
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
Identify the characterization techniques that are based on molecular weights of compounds.
Identify the process by which bacteria protect their own DNA from the attack of restriction enzymes.
Study the following statements regarding separation of DNA using agarose gel electrophoresis.
- Large fragments of DNA molecules move faster than small DNA fragments and thus separate faster.
- DNA being negatively charged migrates to cathode.
Which of the following statements is correct with respect to cellular respiration?
The source of taq Polymerase used in PCR is a ______.
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
How many sets of primers are required in each cycle of PCR?
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.
