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प्रश्न
What is Recognition sequence? Explain in brief.
उत्तर
- The sequences recognized by restriction enzymes are 4 to 8 nucleotides long and characterized by a particular type of internal symmetry is known as recognition sequence.
- When the sequence is read in opposite direction (3’ to 5’ or 5’ to 3’) it is identical/ same. For e.g. consider the particular sequence recognized by the enzyme EcoRI. 3’ -______ CTTAAG______ 5’ 5’______ GAATTC ______ 3’
- Restriction enzymes either cut straight across the DNA in the region of palindrome to give blunt ends or cuts producing short, single stranded projections at each end of DNA to produce, cohesive or sticky ends or staggered ends.
संबंधित प्रश्न
The biological scissor is ______.
What are the restriction endonucleases? Give the rules of their nomenclature.
What is the role of Taq-polymerase in PCR technology?
From which of the following organisms the enzyme Taq polymerase used in PCR technique is obtained?
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
Study the following statements regarding separation of DNA using agarose gel electrophoresis.
- Large fragments of DNA molecules move faster than small DNA fragments and thus separate faster.
- DNA being negatively charged migrates to cathode.
Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
The main reason for the presence of both a leading and a lagging strand during DNA replication is, ______
Which of the following statements is correct with respect to cellular respiration?
Which of the following viruses has the capacity to produce lysozymes?
