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प्रश्न
What is Recognition sequence? Explain in brief.
उत्तर
- The sequences recognized by restriction enzymes are 4 to 8 nucleotides long and characterized by a particular type of internal symmetry is known as recognition sequence.
- When the sequence is read in opposite direction (3’ to 5’ or 5’ to 3’) it is identical/ same. For e.g. consider the particular sequence recognized by the enzyme EcoRI. 3’ -______ CTTAAG______ 5’ 5’______ GAATTC ______ 3’
- Restriction enzymes either cut straight across the DNA in the region of palindrome to give blunt ends or cuts producing short, single stranded projections at each end of DNA to produce, cohesive or sticky ends or staggered ends.
संबंधित प्रश्न
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
Name and explain the technique used for separating DNA fragments and making them available for biotechnology experiments.
Expand the following abbreviation: BAC
Enlist and write in brief about the different biological tools required in rDNA technology.
Ti plasmid being used for introducing genes in plants obtained from ______.
What is gene cloning? Explain different tools used for it.
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
Which of the following statements is correct with respect to cellular respiration?
DNA fingerprinting refers to ______.
