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प्रश्न
State the role of DNA ligase in biotechnology.
उत्तर
DNA ligase enzyme is used to join two DNA fragments from their ends.
APPEARS IN
संबंधित प्रश्न
Write a short note on Electrophoresis.
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
Which molecules are called molecular scissors? Why are they called so?
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is ______.
Write a short note on vectors.
Tissue plasminogen activator and urokinase are used in the treatment of ______.
Name the enzymes that are called molecular scissors.
