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प्रश्न
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
उत्तर
- The given cloning vector is called pBR322 and it is inserted into E. coli bacteria.
- In the image shown, the two restriction sites are Hind III and Bam H I.
- The selectable markers in the cloning vector pBR322 include ampicillin and tetracycline resistance genes.
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संबंधित प्रश्न
The biological scissor is ______.
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
Plasmid is ______.
Explain the mechanism of PCR.
Which one of the following enzymes is used to join DNA fragments?
