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प्रश्न
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
उत्तर
- The given cloning vector is called pBR322 and it is inserted into E. coli bacteria.
- In the image shown, the two restriction sites are Hind III and Bam H I.
- The selectable markers in the cloning vector pBR322 include ampicillin and tetracycline resistance genes.
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संबंधित प्रश्न
Expand the following abbreviation: BAC
Explain the properties of a good or ideal cloning vector for rDNA technology.
From the following list of cloning vectors used in rDNA technology identify the vectors that are the most commonly used.
Plasmids, bacteriophages (Ml3, lambda virus), cosmid, phagemid, BAC (bacterial artificial chromosome), YAC (yeast artificial chromosome), transposons, baculoviruses, and mammalian artificial chromosomes (MACs)
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
Consider there is only one double stranded DNA molecule initially along with all other requirements of PCR. If a PCR machines performs 10 cycles of reaction then bow many double stranded DNA molecules would be present at the end?
A technique used to make numerous copies of a specific DNA segment quickly and accurately is called ______
Name the plant disease caused by Agrobacterium tumefaciens.
The polymerase chain reaction is a powerful technique to ______.
Plasmid is ______.
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
