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प्रश्न
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
विकल्प
Origin of replication (ori)
Presence of a selectable marker
Presence of sites for restriction endonuclease
Its size
उत्तर
The most important feature in a plasmid to serve as a vector in gene cloning experiment is origin of replication (ori).
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संबंधित प्रश्न
Retroviruses have no DNA. However, the DNA of the infected host cell does possess viral DNA. How is it possible?
Write a short note on Electrophoresis.
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
Explain the properties of a good or ideal cloning vector for rDNA technology.
Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
Identify the CORRECT sequence of steps in PCR.
Identify the characterization techniques that are based on molecular weights of compounds.
The main reason for the presence of both a leading and a lagging strand during DNA replication is, ______
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
David is a molecular biologist. He uses a technique to know the amino acids occupy the first and the last positions in the Chain - A and Chain - B of insulin. Name the scientist whose contribution made it possible for David to use this technique.
