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प्रश्न
Explain the properties of a good or ideal cloning vector for rDNA technology.
उत्तर
Following characteristic properties, a cloning vector must possess in order to be used in rDNA technology:
- A good vector should have the ability of independent replication so that as the vector replicates (through ori gene) and a large number of copies of the DNA insert will be formed.
- The vector should be able to easily introduce into host cells.
- A vector should have marker genes for antibiotic resistance.
- A vector must contain a unique cleavage site in one of the marker genes for the restriction enzyme.
- It should have at least suitable control elements like a promoter, operator, ribosomal binding sites, etc.
- The plasmids obtained naturally do not possess all the characteristics. Hence, they are constructed by inserting a gene for antibiotic resistance. eg. pBR322, pBR320, pACYC177 are the constructed plasmids. pBR322 is mostly used in rDNA technology in plants.
संबंधित प्रश्न
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
The enzyme restriction endonuclease ______.
Ti plasmid being used for introducing genes in plants obtained from ______.
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
The main reason for the presence of both a leading and a lagging strand during DNA replication is, ______
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
Which of these are most widely used in genetic engineering?
What is EcoRI?
