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प्रश्न
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
पर्याय
Origin of replication (ori)
Presence of a selectable marker
Presence of sites for restriction endonuclease
Its size
उत्तर
The most important feature in a plasmid to serve as a vector in gene cloning experiment is origin of replication (ori).
APPEARS IN
संबंधित प्रश्न
Give the applications of the PCR technique.
ECoRI is obtained from ______.
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
Match the List I with List II and List ill. Select the correct option.
List I | List II | List ill | |||
i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
iii. | T-DNA | c. | Ampicillin | 3. | Elution |
iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
Which of the following device is required for PCR?
Which molecules are called molecular scissors? Why are they called so?
Name two restriction enzymes used in PCR.
Study the following lists and choose the correct match.
List I | List II |
A. Vector | (i) Resistant to cotton bollworms |
B. Downstream processing | (ii) Mobile genetic elements |
C. Cry II AB | (iii) Controls corn borers |
D. Transposons | (iv) Ti plasmid |
(v) Purifying protein in biopharmaceuticals |
Expand YAC used in the field of Biotechnology.
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.