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प्रश्न
Name two restriction enzymes used in PCR.
उत्तर
- EcoRI
- Hind Ill
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संबंधित प्रश्न
Give an example of the source of thermostable enzyme DNA polymerase.
What are plasmids?
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
The time taken to complete one cycle of PCR is around ______.
______ is a Type I restriction enzyme.
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
Annealing step of PCR operates at ______ °C.
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is ______.
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
