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प्रश्न
Annealing step of PCR operates at ______ °C.
पर्याय
90-98
40-60
70-75
100-120
उत्तर
Annealing step of PCR operates at 40-60 °C.
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संबंधित प्रश्न
How has the development of bioreactor helped in biotechnology?
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
The enzyme restriction endonuclease ______.
For which of the following reason Agrobacterium tumefaciens is most widely used for gene transfer in plants?
Identify the characterization techniques that are based on molecular weights of compounds.
Which of the following device is required for PCR?
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Name two restriction enzymes used in PCR.
