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प्रश्न
Explain the following terms with respect to rDNA technology
- passanger DNA
- Chimeric DNA
- Transformed cell
- restriction site
उत्तर
- Passenger DNA: A DNA fragment containing desired gene that is isolated and selected for cloning is called foreign DNA or passenger DNA.
- Chimeric DNA: The combination of vector DNA and foreign DNA is called Recombinant DNA or Chimeric DNA.
- Transformed cell: The host/ competent cell which has taken up rDNA is called transformed cell.
- Restriction site: The particular sequence of nucleotides on DNA where a restriction enzyme cuts the two strands of DNA is called recognition or restriction site.
संबंधित प्रश्न
Recombinant protein .................. is used to dissolve blood clots present in the body.
Give the role of Ca++ ions in the transfer of the recombinant vector into the bacterial host cells.
In the process of rDNA technology, if two separate restriction enzymes are used to cut vector and donor DNA then which problem will arise in the formation of rDNA or chimeric DNA? Explain.
Explain the steps in process of rDNA technology with suitable diagrams.
Study the following statements regarding cDNA library and select the correct option.
- Study the following statements regarding cDNA library and select the correct option.
- DNA is produced from isolated rRNA by reverse transcription. The DNA so made is called complementary DNA (cDNA).
Study the following statements regarding proteins that are being produced by transgenic crop plants and select the correct option.
i. Human testosterone hormone is produced by gene inserted into the chloroplast DNA of tobacco plants.
ii. Protein antigens to be used in vaccines for e.g. Patient-specific antilymphoma (a cancer) vaccines.
iii. In transgenic plants, Humanized antibodies can be produced against HIV, Respiratory syncytial virus (RSV), Herpes simplex virus (HSY).
Which of the following technique is used for the separation of DNA fragments of different lengths?
Which of the following is used for the isolation and purification of the desired product produced by transformed cells?
Identify the CORRECT statements regarding edible vaccines.
Lambda phage which is commonly used as a cloning vector in rDNA technology usually infects ____________.
