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प्रश्न
Explain the following terms with respect to rDNA technology
- passanger DNA
- Chimeric DNA
- Transformed cell
- restriction site
उत्तर
- Passenger DNA: A DNA fragment containing desired gene that is isolated and selected for cloning is called foreign DNA or passenger DNA.
- Chimeric DNA: The combination of vector DNA and foreign DNA is called Recombinant DNA or Chimeric DNA.
- Transformed cell: The host/ competent cell which has taken up rDNA is called transformed cell.
- Restriction site: The particular sequence of nucleotides on DNA where a restriction enzyme cuts the two strands of DNA is called recognition or restriction site.
संबंधित प्रश्न
cDNA means ______.
Give the role of Ca++ ions in the transfer of the recombinant vector into the bacterial host cells.
In the process of rDNA technology, if two separate restriction enzymes are used to cut vector and donor DNA then which problem will arise in the formation of rDNA or chimeric DNA? Explain.
Explain the steps in process of rDNA technology with suitable diagrams.
Which of the following term represents pairing of primers to the ssDNA segment during PCR?
Which of the following is used for the isolation and purification of the desired product produced by transformed cells?
Identify the process in Column I with the term in Column II and select the correct option.
| Column I | Column II |
| A. Amplification of gene of interest | I. Downstream processing |
| B. Large-scale production of plants in a short duration. | II. Polymerase chain reaction |
| C. Separation and purification of the desired product | III. Transformation |
| D. Insertion of rDNA into the host | IV. Micropropagation |
Define genomic library.
Name the following:
Human protein produced by rDNA technology to treat cancer.
Plasmid DNA containing foreign DNA is called______.
