Advertisements
Advertisements
प्रश्न
In the process of rDNA technology, if two separate restriction enzymes are used to cut vector and donor DNA then which problem will arise in the formation of rDNA or chimeric DNA? Explain.
उत्तर
- Restriction enzymes cut the DNA at a specific recognition site and produce either sticky ends or blunt ends.
- For example, if we use the restriction enzyme EcoRI to cut vector DNA, then it will produce vector DNA having sticky ends, and if we use the Hind II restriction enzyme to cut donor DNA, then it will produce donor DNA with blunt ends.
- A vector DNA to get ligated to the desired gene, they both must possess similar kinds of ends, i.e., both should have either blunt ends or sticky ends.
- If two separate restriction enzymes are used to cut vector and donor DNA, then they will fail to form complementary base pairing, and chimeric DNA will not be formed.
संबंधित प्रश्न
cDNA means ______.
Give the role of Ca++ ions in the transfer of the recombinant vector into the bacterial host cells.
Explain the steps in process of rDNA technology with suitable diagrams.
Explain the following terms with respect to rDNA technology
- passanger DNA
- Chimeric DNA
- Transformed cell
- restriction site
Identify the correct term used to represent the DNA molecule formed by joining a gene coding for insulin to a plasmid pBR322 by using DNA ligase.
Which of the following technique is used for the separation of DNA fragments of different lengths?
Identify the techniques that can be used to transfer foreign DNA directly into the cell or protoplast without the use of plasmids.
i. Biolistic method
ii. Ultrasonification
iii. Shot gun
iv. Lipofection
v. Microinjection
vi. Electroporation
Identify the process in Column I with the term in Column II and select the correct option.
| Column I | Column II |
| A. Amplification of gene of interest | I. Downstream processing |
| B. Large-scale production of plants in a short duration. | II. Polymerase chain reaction |
| C. Separation and purification of the desired product | III. Transformation |
| D. Insertion of rDNA into the host | IV. Micropropagation |
Define genomic library.
Give reason: All the fragments of a genome are cloned for storing them in genomic library.
