Advertisements
Advertisements
Question
Explain the following terms with respect to rDNA technology
- passanger DNA
- Chimeric DNA
- Transformed cell
- restriction site
Solution
- Passenger DNA: A DNA fragment containing desired gene that is isolated and selected for cloning is called foreign DNA or passenger DNA.
- Chimeric DNA: The combination of vector DNA and foreign DNA is called Recombinant DNA or Chimeric DNA.
- Transformed cell: The host/ competent cell which has taken up rDNA is called transformed cell.
- Restriction site: The particular sequence of nucleotides on DNA where a restriction enzyme cuts the two strands of DNA is called recognition or restriction site.
RELATED QUESTIONS
Which of the following is used for assisting the transfer of rDNA into a bacterial cell?
Identify the correct term used to represent the DNA molecule formed by joining a gene coding for insulin to a plasmid pBR322 by using DNA ligase.
Which of the following technique is used for the separation of DNA fragments of different lengths?
Which of the following is/are the CORRECT step/s involved in construction of a recombinant DNA?
A ______ refers to the competent host cell that has taken up chimeric DNA.
Identify the techniques that can be used to transfer foreign DNA directly into the cell or protoplast without the use of plasmids.
i. Biolistic method
ii. Ultrasonification
iii. Shot gun
iv. Lipofection
v. Microinjection
vi. Electroporation
Identify the CORRECT statements regarding edible vaccines.
Give reason: All the fragments of a genome are cloned for storing them in genomic library.
Name the following:
Human protein produced by rDNA technology to treat cancer.
Plasmid DNA containing foreign DNA is called______.
