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कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

Explain briefly: Chitinase - Biology

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प्रश्न

Explain briefly:

Chitinase

थोडक्यात उत्तर

उत्तर

Chitinase is a class of enzymes used for the degradation of chitin, which forms a significant component of the fungal cell wall. Therefore, to isolate the DNA enclosed within the cell membrane of the fungus, the enzyme chitinase is used to break the cell to release its genetic material.

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Processes of Recombinant DNA Technology
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पाठ 11: Biotechnology : Principles and Processes - Exercises [पृष्ठ २०६]

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एनसीईआरटी Biology [English] Class 12
पाठ 11 Biotechnology : Principles and Processes
Exercises | Q 11.3 | पृष्ठ २०६

संबंधित प्रश्‍न

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?


Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?


Can you recall meiosis and indicate at what stage a recombinant DNA is made?


Describe briefly the following:

Bioreactors


Explain briefly:

PCR


Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.


Answer the following question:
Describe the process of amplification of the "gene of interest" using the PCR technique.


Which of the following term used for defining a viral genome incorporated into host DNA?


PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.


During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.


During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?


Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?


Significance of 'heat shock' method in bacterial transformation is to facilitate ______.


A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be ______.


How is copy number of the plasmid vector related to yield of recombinant protein?


Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.


Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.


State the advantage of using Thermostable DNA polymerase.


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