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प्रश्न
Describe briefly the following:
Bioreactors
उत्तर
Bioreactors can be thought of as vessels in which raw materials are biologically converted into specific products, individual enzymes, etc., using microbial plant, animal, or human cells. A bioreactor provides the optimal conditions for achieving the desired product by providing optimum growth conditions (temperature, pH, substrate, salts, vitamins, oxygen). The most commonly used bioreactor is the stirring type. Stirred-tank reactors are usually cylindrical or have a curved base.
This helps mix the material inside the reactor. Stirrers not only mix the material inside the reactor but also ensure the availability of oxygen everywhere in the reactor. Every bioreactor has an agitator system. It also has an oxygen delivery system, foam control system, temperature control system, and pH control device. The reaction is controlled by a system and sampling, which allows small volumes of the cultured product to be periodically removed.
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संबंधित प्रश्न
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
Can you recall meiosis and indicate at what stage a recombinant DNA is made?
Describe briefly the following:
Downstream processing
Explain briefly:
PCR
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
Answer the following question:
Describe the process of amplification of the "gene of interest" using the PCR technique.
DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by ______.
A single strand of nucleic acid tagged with a radioactive molecule is called ______.
Gene Amplification using primers can be done by ______.
During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.
Rising of dough is due to ______.
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
How is copy number of the plasmid vector related to yield of recombinant protein?
Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.
Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.
