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प्रश्न
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
पर्याय
Laboratory flask of largest capacity
A stirred-tank bioreactor without in-lets and out-lets
A continuous culture system
Any of the above
उत्तर
A continuous culture system
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संबंधित प्रश्न
Can you recall meiosis and indicate at what stage a recombinant DNA is made?
Explain briefly:
PCR
State the importance of using a bioreactor.
DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by ______.
Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
How is copy number of the plasmid vector related to yield of recombinant protein?
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?
Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.
Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.
Answer the following questions w.r.t. the given paragraph:
- List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
- Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
- Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.
