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प्रश्न
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
उत्तर
If denaturation of double-stranded DNA does not take place, then primers will not be able to anneal to the template, no extension will take place, hence no amplification will occur.
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संबंधित प्रश्न
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Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
