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प्रश्न
Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
पर्याय
Both (A) and (R) are true and (R) is the correct explanation of (A)
Both (A) and (R) are true, but (R) is not the correct explanation of (A)
(A) is true, but (R) is false
(A) is false, but (R) is true
उत्तर
Both (A) and (R) are true, but (R) is the correct explanation of (A)
Explanation:
In order to base-pair with a strand of DNA or RNA, oligonucleotides are created. Oligonucleotides are most frequently used as polymerase chain reaction primers. The 5' end of the sequence intended for amplification serves as the complement to at least part of the sequence used in the construction of primers. The primers anneal to the complementary sequences on the DNA templates during the annealing process.
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Read the paragraph given below and answer and questions that follow:
| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
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