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प्रश्न
In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.
उत्तर
The flow chart shows the three steps involved in the process of PCR showing the following -
- Denaturation The DNA strands are treated with a temperature of 94°C (Heat) and the strands are separated.
- Annealing The primers anneal to the complementary strands
- Extension The DNA polymerase facilitates the extension of the strands.
OR
Polymerase Chain Reaction
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संबंधित प्रश्न
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
Can you recall meiosis and indicate at what stage a recombinant DNA is made?
Describe briefly the following:
Bioreactors
Explain briefly:
PCR
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
______ plays an important role in blood clotting.
Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
State the advantage of using Thermostable DNA polymerase.
Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.
Answer the following questions w.r.t. the given paragraph:
- List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
- Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
- Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.
