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प्रश्न
Who among the following was awarded the Nobel Prize for the development of PCR technique?
विकल्प
Herbert Boyer
Hargovind Khurana
Kary Mullis
Arthur Kornberg
उत्तर
Kary Mullis
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संबंधित प्रश्न
What are plasmids?
Name and explain the technique used for separating DNA fragments and making them available for biotechnology experiments.
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
What is plasmid?
Define biotechnology? Which are the basic principles and process of biotechnology?
The PCR technique is used for ______.
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
Write steps involved in gene amplification.
David is a molecular biologist. He uses a technique to know the amino acids occupy the first and the last positions in the Chain - A and Chain - B of insulin. Name the scientist whose contribution made it possible for David to use this technique.
