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Question
Describe briefly the following:
Origin of replication
Solution
One of the most important components of a plasmid is the sequence of bases that initiates replication. It is known as the origin of replication (ori). This is a region of the plasmid genome that acts as a start signal for self-replication (making another copy of itself). Any portion of DNA connected to this sequence can be replicated within host cells. This characteristic is utilised to create multiple copies of linked DNA (or DNA inserts).
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RELATED QUESTIONS
Name the selectable markers in the cloning vector pBR322? Mention the role they play.
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(c) An antibiotic resistant gene.
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A clone is a ______.
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- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
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The CORRECT combination is
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The figure below shows the structure of a plasmid.
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