Advertisements
Advertisements
Question
What does ‘competent’ refer to in competent cells used in transformation experiments?
Solution
Competent means bacterial cells, on treatment with CaCl2, are made capable of taking up foreign DNA.
APPEARS IN
RELATED QUESTIONS
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
How does the β-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain.
The genetic material of virus includes:
Which of the following is correctly matched?
Which of the following statement is not true for a clone?
Which of the following is not a cloning vector?
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
