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प्रश्न
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
उत्तर
Importance of Selectable Marker:-
(i) It helps in identifying and eliminating non-transformants.
(ii) It selectively permitting the growth of the transformant.
e.g- The genes encoding resistance to antibiotics such as Ampicillin, Chloramphenicol Tetracyclin or Kanamycin are considered useful selectable markers for E.coli because normally E-coli does not carry resistance against any of these antibiotics.
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संबंधित प्रश्न
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
Describe briefly the following:
Origin of replication
Why is the coding sequence of an enzyme β - galactosidase a preferred selectable marker in comparison to the ones named above?
The genetic material of virus includes:
A clone is a ______.
Which of the following statement is not true for a clone?
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
Which of the following is not a cloning vector?
Name the regions marked A, B and C.
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
