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प्रश्न
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
उत्तर
pBR322
The Restriction sites mentioned in the figure are Hind III, EcoR I, BamH I, Sal I, Pvu II, Pst I, Cla I (any two can be considered) Antibiotic resistant genes: ampR and tetR
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संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322? Mention the role they play.
Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
What is ‘Ori’ ? State its importance during cloning of a vector.
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
The genetic material of virus includes:
Which of the following is correctly matched?
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
What does ‘competent’ refer to in competent cells used in transformation experiments?
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
Name the regions marked A, B and C.
