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प्रश्न
Write the role of Ori and 'restriction' site in a cloning vector pBR322.
उत्तर
Ori- It is a genetic sequence that acts as the initiation site for replication of DNA. Any fragment of DNA, when linked to the ori region, can be initiated to replicate.
Restriction site- It is the recognition site for restriction enzymes (such as EcoRI, Hind III, PvuI, BamHI, etc.). Recognition sites are the genetic sequences from where the restriction enzymes cut the DNA.
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संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322? Mention the role they play.
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
Why is the coding sequence of an enzyme β - galactosidase a preferred selectable marker in comparison to the ones named above?
How does the β-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain.
The genetic material of virus includes:
A suitable vector for gene cloning in higher organism is ______.
Which of the following is not a cloning vector?
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain.
Describe the role of CaCl2 in the preparation of competent cells?
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
