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प्रश्न
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
पर्याय
Medium with Tetracycline Medium with Ampicillin Growth No growth Medium with Tetracycline Medium with Ampicillin No growth Growth Medium with Tetracycline Medium with Ampicillin No growth No growth Medium with Tetracycline Medium with Ampicillin Growth Growth
उत्तर
| Medium with Tetracycline | Medium with Ampicillin |
| No growth | Growth |
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संबंधित प्रश्न
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
Why is the coding sequence of an enzyme β - galactosidase a preferred selectable marker in comparison to the ones named above?
Write the role of Ori and 'restriction' site in a cloning vector pBR322.
What is ‘Ori’ ? State its importance during cloning of a vector.
How does the β-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain.
A suitable vector for gene cloning in higher organism is ______.
Which of the following is not a genetic vector?
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
What does ‘competent’ refer to in competent cells used in transformation experiments?
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
