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प्रश्न
Why must a cell be made ‘competent’ in biotechnology experiments? How does calcium ion help in doing so?
उत्तर
In biotechnology experiments, the cells must be rendered competent to enable them to absorb the hydrophilic DNA molecule from the external medium. Divalent calcium cations are administered to bacterial cells to render them competent and facilitate the uptake of DNA through the apertures in the cell wall.
संबंधित प्रश्न
Mention the type of host cells suitable for the gene guns to introduce an alien DNA.
Write the steps you would suggest to be undertaken to obtain a foreign-gene-product.
State the role of “biolistic gun” in biotechnology experiments.
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Plasmids
Why should a bacterium be made ‘competent’ ?
Explain the role of ‘microinjection’ and ‘gene gun’ in biotechnology
For transformation, micro particles coated with DNA to be bombarded with gene gun are made up of ______.
Non viral and non vector methods are sometimes used to transfer genes or alien DNA into a plant cell. Explain one such method used in genetic engineering.
