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प्रश्न
Arrange the following steps of DNA fingerprinting in correct sequence:
- Gel electrophoresis
- Isolation of DNA
- Southern blotting
- Restriction digestion
उत्तर
b. Isolation of DNA
d. Restriction digestion
a. Gel electrophoresis
c. Southern blotting
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संबंधित प्रश्न
State the best-known contribution of Alec Jeffery
Short Answer Question:
Match the entries in column I with those of column II and choose the correct answer.
| Column I | Column II |
| A. Alkali treatment | i. Separation of DNA fragments on gel slab |
| B. Southern blotting | ii. Split DNA fragments into single strands |
| C. Electrophoresis | iii. DNA transferred to nitrocellulose sheet |
| D. PCR | iv. X-ray photography |
| E. Autoradiography | v. Produce fragments of different sizes |
| F. DNA treated with REN | vi. DNA amplification |
Define 'RFLP’.
Assertion: rDNA is superior over hybridisation techniques.
Reason: Desired genes are inserted without introducing the undesriable genes in target organisms.
State the applications of DNA fingerprinting technique.
ln which of the following technique DNA bands are transferred from agarose gel to nitrocellulose or nylon membrane?
Which of the following result is obtained when DNA sample hybridized with DNA probe is kept in contact with X-ray film?
____________ paper is used for embedding DNA strands in Southern blotting technique.
List the various markers that are used in DNA finger printing.
DNA fragments formed by the action of restriction enzyme in a chemical reaction can be separated by ______.
