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Question
Arrange the following steps of DNA fingerprinting in correct sequence:
- Gel electrophoresis
- Isolation of DNA
- Southern blotting
- Restriction digestion
Solution
b. Isolation of DNA
d. Restriction digestion
a. Gel electrophoresis
c. Southern blotting
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Polymorphism
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State the best-known contribution of Alec Jeffery
Short Answer Question:
Match the entries in column I with those of column II and choose the correct answer.
| Column I | Column II |
| A. Alkali treatment | i. Separation of DNA fragments on gel slab |
| B. Southern blotting | ii. Split DNA fragments into single strands |
| C. Electrophoresis | iii. DNA transferred to nitrocellulose sheet |
| D. PCR | iv. X-ray photography |
| E. Autoradiography | v. Produce fragments of different sizes |
| F. DNA treated with REN | vi. DNA amplification |
What is the use of southern blotting in DNA fingerprinting?
DNA fingerprinting is based on the principle of identifying______.
Assertion: rDNA is superior over hybridisation techniques.
Reason: Desired genes are inserted without introducing the undesriable genes in target organisms.
VNTR has ______.
The process in which sample DNA and probe DNA form double stranded DNA is called ____________.
Which of the following is not true with respect to INA fingerprinting?
- DNA fingerprinting technique was first developed by Alec Jeffrey.
- DNA profiling is a more recent and more sensitive version of DNA fingerprinting.
- DNA fingerprinting was first used in Britain to verify the parentage of an immigrant, who had left the country and then wanted to return
- In DNA fingerprinting, single stranded radioactive DNA is used as probes.
Choose the correct option.
