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प्रश्न
Attempt any TWO of the following:
With the help of a suitable example illustrate ‘Palindrome’.
उत्तर
A palindromic sequence is a sequence made up of nucleic acids within double helix of DNA and/or RNA that is the same when read from 5’ to 3’ on one strand and 5’ to 3’ on the other, complementary, strand. It is also known as a palindrome or an inverted-reverse sequence.
The pairing of nucleotides within the DNA double-helix is complementary which consist of Adenine (A) pairing with either Thymine (T) in DNA or Uracil (U) in RNA, while Cytosine (C) pairs with Guanine (G). So if a sequence is palindromic, the nucleotide sequence of one strand would be the same as its reverse complementary strand. An example of a palindromic sequence is 5’-GGATCC-3’, which has a complementary strand, 3’-CCTAGG-5’. This is the sequence where the restriction endonuclease, BamHI, binds to and cleaves at a specific cleavage site. When the complementary strand is read backwards, the sequence is 5’-GGATCC-3’ which is identical to the first one, making it a palindromic sequence.
Another restriction enzyme called EcoR1 recognizes and cleaves the following palindromic sequence:
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संबंधित प्रश्न
Very Short Answer Question:
Which are the nucleosomal 'core' histones?
Differentiate between Heterochromatin & Euchromatin.
Explain the formation of a nucleosome.
The additional set of proteins required for packaging of chromatin at higher level are called ______.
Find out the wrong statement about heterochromatin.
A stretch of euchromatin has 200 nucleosomes. How many bp will there be in the stretch and what would be the length of the typical euchromatin?
Read the following and answer from given below:
In prokaryotes, DNA is circular and present in the cytoplasm but in eukaryotes, DNA is linear and mainly confined to the nucleus. DNA or deoxyribonucleic acid is a long polymer of nucleotides. In 1953, the first correct double-helical structure of DNA was worked out by Watson and Crick. Based on the X-ray diffraction data produced by Maurice Wilkins and Rosalind Franklin. It is composed of three components, i.e., A phosphate group, a deoxyribose sugar, and a nitrogenous base. Different forms of DNA are B-DNA, Z-DNA, A-DNA, C-DNA, and D-DNA.
The double chain of B-DNA is coiled in a helical fashion. The spiral twisting of the B-DNA duplex produces ______
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Electron Transport System (ETS) is present in a eukaryotic cell in the:
In an experiment, DNA is treated with a compound which tends to place itself amongst the stacks of nitrogenous base pairs. As a result of which, the distance between two consecutive base increases, from 0.34nm to 0.44 nm. Calculate the length of DNA double helix (which has 2 × 109 bp) in the presence of saturating amount of this compound.
