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प्रश्न
Comment on the utility of variability in number of tandem repeats during DNA finger printing.
उत्तर
Tandemness in repeats provides many copies of the sequence for fingerprinting, and variability in nitrogen base sequence in them. Being individual-specific, this proves to be useful in the process of DNA fingerprinting.
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संबंधित प्रश्न
Which material is used for isolation of DNA in fingerprinting technique?
Short Answer Question:
Match the entries in column I with those of column II and choose the correct answer.
| Column I | Column II |
| A. Alkali treatment | i. Separation of DNA fragments on gel slab |
| B. Southern blotting | ii. Split DNA fragments into single strands |
| C. Electrophoresis | iii. DNA transferred to nitrocellulose sheet |
| D. PCR | iv. X-ray photography |
| E. Autoradiography | v. Produce fragments of different sizes |
| F. DNA treated with REN | vi. DNA amplification |
What is the use of southern blotting in DNA fingerprinting?
Assertion: rDNA is superior over hybridisation techniques.
Reason: Desired genes are inserted without introducing the undesriable genes in target organisms.
Identify the CORRECT sequence of steps involved DNA fingerprinting.
i. Isolation of genomic DNA
ii. Separation by electrophoresis
iii. Denaturation of DNA
iv. Hybridization with probe
v. Fragmenting DNA with restriction enzymes
vi. Southern Blotting
vii. Exposure on film
In DNA fingerprinting technique, the radioactive intermediates formed during hybridization are______ molecules.
Identify the criterion for movement of DNA fragments on agarose gel during gel electrophoresis.
The image below depicts the result of gel electrophoresis -
If the ladder represents sequence length upto 3000 base pairs (bp),
- Which of the bands (I - IV) correspond to 2500 bp and 100 bp respectively?
- Explain the basis of this kind of separation and also mention the significance of this process.
DNA fingerprinting involves identifying differences in some specific regions in DNA sequence, called as ______
What is DNA polymorphism? Why is it important to study it?
