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प्रश्न
Differentiate between the following:
Repetitive DNA and Satellite DNA
उत्तर
| Repetitive DNA | Satellite DNA |
| It is the section of DNA that contains the same base sequences repeated numerous times. The repeating sequences in this repetitive DNA might be tandem or interspersed repeatedly. | The portion of repetitive DNA contains repeated sequences in tandem, also known as tandemly repetitive DNA. |
| Repeating units can be a few base pairs long or hundreds to thousands of base pairs long. | It has small sequence repetitions (up to 60 bp long). |
| They appear as bright bands in the CsCl density gradient study. | In CsCl density gradient analysis, it is seen as small dark bands. |
| During density gradient centrifugation, repetitive DNAs are isolated from bulk genome DNA by distinct peaks. | It is separated from repetitive DNA as a satellite. Satellite DNA has been divided into small peaks. |
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संबंधित प्रश्न
Write down the full form of R.F.L.P.
The genetic marker used as key factor in DNA fingerprinting is _______.
(A) VNTRs
(B) Exons
(C) Introns
(D) DNA probes
Explain the significance of satellite DNA in DNA fingerprinting technique.
"A very small sample of tissue or even a drop of blood can help determine paternity." Provide a scientific explanation to substantiate how it is possible.
What is the principle of DNA profiling?
Similar DNA fingerprinting is obtained for______.
Assertion: rDNA is superior over hybridisation techniques.
Reason: Desired genes are inserted without introducing the undesriable genes in target organisms.
Which of the following are the applications of DNA fingerprinting?
Which of the following process is carried out by restriction endonuclease used in DNA fingerprinting?
Identify the CORRECT description of DNA fingerprinting.
Which of the following is the technique developed to identify a person with the help of DNA restriction analysis?
DNA having variable length obtained by restriction digestion is called ______.
The process in which sample DNA and probe DNA form double stranded DNA is called ____________.
Match the Column - I with Column - II and select the correct option
| Column - I | Column - II | ||
| (a) | Female banded Krait | (i) | Humulin |
| (b) | Pancreas from dog | (ii) | DNA sample |
| (c) | Hair root of human | (iii) | Insulin extracted and purified |
| (d) | E.coli | (iv) | DNA probe |
Which one of the following is an incorrect statement with regard to pedigree analysis?
Would it be appropriate to use DNA probes such as VNTR in DNA finger printing of a bacteriaphage?
‘There is a paternity dispute for a child’. Which technique can solve the problem. Discuss the principle involved.
Match the following pairs:
| Column I | Column II | ||
| (i) | PCR | (a) | DNA transferred to nylon membrane |
| (ii) | Electrophoresis | (b) | DNA amplification |
| (iii) | Southern blotting | (c) | Restriction digestion |
| (iv) | Autoradiography | (d) | Produce fragments of different size |
| (v) | Alkali treatment | (e) | Separation of DNA fragments on gel slab |
| (vi) | DNA treated with REN | (f) | X-ray photography |
Enlist the steps in DNA fingerprinting giving its applications.
