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प्रश्न
You are repeating the Hershey-Chase experiment and are provided with two isotopes: 32P and 15N (in place of 35S in the original experiment). How do you expect your results to be different?
उत्तर
Use of 15N will be inappropriate because the method of detection of 35p and 15N is different (32p being a radioactive isotope while 15N is not radioactive but is the heavier isotope of Nitrogen). Even if 15N was radioactive then its presence would have been detected both inside the cell (15N incorporated as nitrogenous base in DNA) as well as in the supernatant because 15N would also get incorporated in the amino group of amino acids in proteins). Hence the use of 15N would not give any conclusive results.
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संबंधित प्रश्न
How did Hershey and Chase differentiate between DNA and protein in their experiment while proving that DNA is the genetic material?
Answer the following question.
Explain the mechanism of DNA replication with the help of a replication fork. What role does the enzyme ONA-ligase play in a DNA replication fork?
When DNA is transferred from a culture of capsulated Streptococcus to a culture of non-capsulated Streptococcus, converting the latter into the former type, the process is known as ____________.
Identify the disease which is NOT transmitted through contaminated food and water.
Hershey and Chase proved that genetic material is DNA, by using the organism ______
A DNA with unequal nitrogen bases would most probably be ______.
Oswald Avery, Colin MacLeod, and Maclyn McCarty used enzymes to purify biochemicals such as proteins, DNA, and RNA from the heat-killed S cells to see which ones could transform live R cells into S cells in Griffith's experiment. They observed that ______
Alfred Hershey and Martha Chase used ______ to provide unequivocal proof that DNA is the genetic material.
Classify the following to form Column B as per the category given m Column A:
(i) UUU, (ii) CUA, (iii) UAA, (iv) AUG, (v) UAG, (vi) UGA
| Column 'A' | Column 'B' | |
| (1) | Initiator codon | |
| (2) | Stop codons | |
| (3) | Codon that codes for phenylalanin | |
| (4) | Codon that codes for leucine |
Give reason: On injecting a mixture of heat-killed S-bacteria and live R-bacteria, the mice died.
