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प्रश्न
“A very small sample of tissue or even a drop of blood can help determine paternity”. Provide a scientific explanation to substantiate the statement.
उत्तर
A very small sample of tissue or even a drop of blood can help determine paternity by DNA fingerprinting. The DNA is extracted from the nuclei of cells or blood cell. The DNA molecules are first broken with the help of an enzyme restriction endonuclease which cuts them into fragments which contain variable number tandem repeats (VNTRs). The fragments are separated according to size by gel electrophoresis and are multiplied by the polymerase chain reaction (PCR) technique. They are then treated with alkaline chemicals to split them into single-stranded DNAs and are transferred onto a nylon membrane. An X-ray film is exposed to the nylon membrane on which the dark bands represent the DNA fingerprints.
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संबंधित प्रश्न
In DNA fingerprinting technique, radioactive DNA probe is obtained from______ of female banded krait snake.
(a) X chromosome
(b) Y chromoson1e
(c) X and Y chromosomes
(d) autosome
Briefly describe the technique employed in DNA fingerprinting.
Short Answer Question:
Write a note on applications of DNA fingerprinting.
What is the use of southern blotting in DNA fingerprinting?
In DNA fingerprinting technique, which of the following probe is used for hybridization of DNA fragments?
DNA having variable length obtained by restriction digestion is called ______.
Short stretches of DNA used to identify the complementary sequences in a sample are called ______
Which one of the following is an incorrect statement with regard to pedigree analysis?
Name and describe the steps involved in the technique widely used in forensics that serves as the basis of paternity testing in case of disputes.
Enlist the steps in DNA fingerprinting giving its applications.
