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प्रश्न
Expand VNTR and describe its role in DNA fingerprinting.
उत्तर १
VNTR: Variable Number of Tandem Repeats.
DNA fingerprinting involves identifying differences in the number of some specific short nucleotide repeats, called VNTR, that vary in number from person to person and are inherited.
उत्तर २
VNTR stands for “Variable Number of Tandem Repeats”.
The VNTR belongs to a class of satellite DNA referred to as mini-satellite. A small DNA sequence is arranged tandemly in many copy numbers. The copy number varies from chromosome to chromosome in an individual. The numbers of the repeat show very high degree of polymorphism. As a result, the size of VNTR varies in size from 0.1 to 20 kb. Consequently, after hybridization with VNTR probe, the autoradiogram gives many bands of differing sizes. These bands give the characteristic pattern for an individual DNA which is used to identify individuals.
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संबंधित प्रश्न
Long Answer Question:
Guess (i) the possible location of DNA on the collected evidence from a crime scene and (ii) the possible sources of DNA.
| Evidence | Possible location of DNA on the evidence | Sources of DNA |
| e.g. Eyeglasses | e.g. Ear pieces | e.g. Sweat, Skin |
| Bottle, Can, Glass | Sides, mouthpiece | __________ |
| __________ | Handle | Sweat, skin, blood |
| Used cigarette | Cigarette butt | ____________ |
| Bite mark | ____________ | Saliva |
| __________ | Surface area | Hair, semen, sweet, urine |
Which of the following is the technique developed to identify a person with the help of DNA restriction analysis?
Which of the following result is obtained when DNA sample hybridized with DNA probe is kept in contact with X-ray film?
____________ paper is used for embedding DNA strands in Southern blotting technique.
Match the Column - I with Column - II and select the correct option
| Column - I | Column - II | ||
| (a) | Female banded Krait | (i) | Humulin |
| (b) | Pancreas from dog | (ii) | DNA sample |
| (c) | Hair root of human | (iii) | Insulin extracted and purified |
| (d) | E.coli | (iv) | DNA probe |
Polymorphism arises due to ______
Which of the following technique is used m DNA fingerprinting?
Would it be appropriate to use DNA probes such as VNTR in DNA finger printing of a bacteriaphage?
Match the following pairs:
| Column I | Column II | ||
| (i) | PCR | (a) | DNA transferred to nylon membrane |
| (ii) | Electrophoresis | (b) | DNA amplification |
| (iii) | Southern blotting | (c) | Restriction digestion |
| (iv) | Autoradiography | (d) | Produce fragments of different size |
| (v) | Alkali treatment | (e) | Separation of DNA fragments on gel slab |
| (vi) | DNA treated with REN | (f) | X-ray photography |
Arrange the following steps of DNA fingerprinting in correct sequence:
- Gel electrophoresis
- Isolation of DNA
- Southern blotting
- Restriction digestion
