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प्रश्न
Expand VNTR and describe its role in DNA fingerprinting.
उत्तर १
VNTR: Variable Number of Tandem Repeats.
DNA fingerprinting involves identifying differences in the number of some specific short nucleotide repeats, called VNTR, that vary in number from person to person and are inherited.
उत्तर २
VNTR stands for “Variable Number of Tandem Repeats”.
The VNTR belongs to a class of satellite DNA referred to as mini-satellite. A small DNA sequence is arranged tandemly in many copy numbers. The copy number varies from chromosome to chromosome in an individual. The numbers of the repeat show very high degree of polymorphism. As a result, the size of VNTR varies in size from 0.1 to 20 kb. Consequently, after hybridization with VNTR probe, the autoradiogram gives many bands of differing sizes. These bands give the characteristic pattern for an individual DNA which is used to identify individuals.
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संबंधित प्रश्न
The genetic marker used as key factor in DNA fingerprinting is _______.
(A) VNTRs
(B) Exons
(C) Introns
(D) DNA probes
Following the collision of two trains a large number of passengers are killed. A majority of them are beyond recognition. Authorities want to hand over the dead to their relatives. Name a modern scientific method and write the procedure that would help in the identification of kinship.
What is DNA fingerprinting?
State the best-known contribution of Alec Jeffery
ln DNA profiling technique, the nitrocellulose paper is used in the process called ____________.
Match the Column - I with Column - II and select the correct option
| Column - I | Column - II | ||
| (a) | Female banded Krait | (i) | Humulin |
| (b) | Pancreas from dog | (ii) | DNA sample |
| (c) | Hair root of human | (iii) | Insulin extracted and purified |
| (d) | E.coli | (iv) | DNA probe |
Which one of the following is an incorrect statement with regard to pedigree analysis?
Polymorphism arises due to ______
Would it be appropriate to use DNA probes such as VNTR in DNA finger printing of a bacteriaphage?
Arrange the following steps of DNA fingerprinting in correct sequence:
- Gel electrophoresis
- Isolation of DNA
- Southern blotting
- Restriction digestion
