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प्रश्न
Following the collision of two trains a large number of passengers are killed. A majority of them are beyond recognition. Authorities want to hand over the dead to their relatives. Name a modern scientific method and write the procedure that would help in the identification of kinship.
उत्तर
DNA fingerprinting is the modern scientific method used for the identification of kinship.
Procedure of DNA Fingerprinting
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Variable Number of Tandem Repeats (VNTR) are satellite DNAs that show high degree of polymorphism. They are used as probes in DNA fingerprinting.
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Firstly, DNA from an individual is isolated and cut with restriction endonucleases.
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Fragments are separated according to their size and molecular weight through gel electrophoresis.
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Fragments separated through electrophoresis gel are blotted (immobilised) on a synthetic membrane such as nylon or nitrocellulose.
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Immobilised fragments are hybridised with a VNTR probe.
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Hybridised DNA fragments can be detected by autoradiography.
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VNTRs vary in size, ranging from 0.1 to 20 kb. Hence, in the autoradiogram, a band of different sizes will be obtained.
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These bands are characteristics of an individual. They are different in each individual, except in the case of identical twins.
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संबंधित प्रश्न
Write down the full form of R.F.L.P.
Give the applications of DNA fingerprinting technique.
Explain the significance of satellite DNA in DNA fingerprinting technique.
Expand VNTR and describe its role in DNA fingerprinting.
How is repetitive/satellite DNA separated from bulk genomic DNA for various genetic experiments?
In DNA fingerprinting technique, which of the following probe is used for hybridization of DNA fragments?
In DNA fingerprinting technique, the radioactive intermediates formed during hybridization are______ molecules.
The DNA fingerprinting pattern of the child is ______
Comment on the utility of variability in number of tandem repeats during DNA finger printing.
Which of the following is not true with respect to INA fingerprinting?
- DNA fingerprinting technique was first developed by Alec Jeffrey.
- DNA profiling is a more recent and more sensitive version of DNA fingerprinting.
- DNA fingerprinting was first used in Britain to verify the parentage of an immigrant, who had left the country and then wanted to return
- In DNA fingerprinting, single stranded radioactive DNA is used as probes.
Choose the correct option.
