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प्रश्न
Give one significant difference between:
Electroporation and Gene Gun.
उत्तर
Electroporation: A method for increasing DNA uptake by protoplast through prior exposure to high voltage which results in the temporary formation of small pore in cell membranes.
Gene Gun: It is the vectorless transfer of gene where tungsten or gold particles coating with desired genes are bombarded into the cells with great force.
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संबंधित प्रश्न
Write a short note on Electrophoresis.
Enlist and write in brief about the different biological tools required in rDNA technology.
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
For which of the following reason Agrobacterium tumefaciens is most widely used for gene transfer in plants?
The time taken to complete one cycle of PCR is around ______.
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
Which of the following is not required in the preparation of a recombinant DNA molecule?
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
What is EcoRI?
