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प्रश्न
How has the use of Agrobacterium as vectors helped in controlling Meloidegyne incognitia infestation in tobacco plants? Explain in correct sequence.
उत्तर
The following steps were taken utilizing Agrobacterium as a transforming vector for tobacco plants to make them immune against the infestation of a nematode Meloidegyne incognitia:
1) Suitable plasmid vectors are chosen and the specific nematode gene is inserted into it.
2) The recombinant plasmids are introduced into the competent Agrobacterium cells.
3) The transformed Agrobacterium cells are screened and selected to infect the tobacco plants.
4) Agrobacterium infection on the tobacco plant cultures leads to insertion and integration of nematode-desired DNA fragment within the tobacco plant.
5) Correct insertion of nematode gene into the host tobacco plants leads to silencing of a essential housekeeping gene of the Meloidegyne incognitia pest by RNAi.
संबंधित प्रश्न
Answer the following questions based on Hershey and Chases's experiments:
(a) Name the kind of virus they worked with and why?
(b) Why did they use two types of culture media to grow viruses in ? Explain.
(c) What was the need for using a blender and later a centrifuge during their experiments?
(d) State the conclusion drawn by them after the experiments.
Give any two features of Genetic Code.
Define Frameshift mutations
(a) Why did Hershey and Chase use radioactive 32P and 35S in their experiments? Explain.
(b) Following the experiments conducted by them, write what conclusion did they arrive at and how ?
The two strands of DNA are held together by ______.
The scientists involved in discovery of DNA as chemical basis of heredity were ______.
Which one is a hereditary disease?
Which of the following methods of reproduction in bacteria involves participation of a virus?
In Griffith's transforming principle, if heat killed S - strain is injected into mice, then ______.
Name the scientist who proved experimentally that DNA is the genetic material ______.
