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प्रश्न
How will you identify a vectors?
थोडक्यात उत्तर
उत्तर
- Vectors are able to replicate autonomously to produce multiple copies of them along with their DNA insert in the host cell.
- It should be small in size and of low molecular weight, less than 10 Kb (kilo base pair) in size so that entry/transfer into the host cell is easy.
- The vector must contain an origin of replication so that it can independently replicate within the host.
- It should contain a suitable marker such as antibiotic resistance, to permit its detection in transformed host cells.
- The vector should have unique target sites for integration with DNA insert and should have the ability to integrate with the DNA insert it carries into the genome of the host cell. Most of the commonly used cloning vectors have more than one restriction site. These are Multiple Cloning Sites (MCS) or polylinker. The presence of MCS facilitates the use of the restriction enzymes of choice.
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Tools for Genetic Engineering
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संबंधित प्रश्न
Restriction enzymes are
Consider the following statements:
- Recombinant DNA technology is popularly known as genetic engineering is a stream of biotechnology which deals with the manipulation of genetic materials by man invitro
- pBR322 is the first artificial cloning vector developed in 1977 by Boliver and Rodriguez from E.coli plasmid
- Restriction enzymes belong to a class
of enzymes called nucleases. Choose the correct option regarding the above statements
Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzymes?
What are the enzymes you can use to cut the terminal end and internal phospho di ester bond of nucleotide sequence?
Name the chemicals used in gene transfer.
What is a restriction enzyme? Mention their type with a role in Biotechnology.
