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Question
How will you identify a vectors?
Answer in Brief
Solution
- Vectors are able to replicate autonomously to produce multiple copies of them along with their DNA insert in the host cell.
- It should be small in size and of low molecular weight, less than 10 Kb (kilo base pair) in size so that entry/transfer into the host cell is easy.
- The vector must contain an origin of replication so that it can independently replicate within the host.
- It should contain a suitable marker such as antibiotic resistance, to permit its detection in transformed host cells.
- The vector should have unique target sites for integration with DNA insert and should have the ability to integrate with the DNA insert it carries into the genome of the host cell. Most of the commonly used cloning vectors have more than one restriction site. These are Multiple Cloning Sites (MCS) or polylinker. The presence of MCS facilitates the use of the restriction enzymes of choice.
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Tools for Genetic Engineering
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