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Chapters
2: Classical Genetics
3: Chromosomal Basis of Inheritance
▶ 4: Principles and Processes of Biotechnology
5: Plant Tissue Culture
6: Principles of Ecology
7: Ecosystem
8: Environmental Issues
9: Plant Breeding
10: Economically Useful Plants and Entrepreneurial Botany
![Samacheer Kalvi solutions for Botany [English] Class 12 TN Board chapter 4 - Principles and Processes of Biotechnology - Shaalaa.com](/images/botany-english-class-12-tn-board_6:5f2b1b2038084cf381bfa42c826a928c.jpg "Samacheer Kalvi solutions for Botany [English] Class 12 TN Board chapter 4 - Principles and Processes of Biotechnology")
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Solutions for Chapter 4: Principles and Processes of Biotechnology
Below listed, you can find solutions for Chapter 4 of Tamil Nadu Board of Secondary Education Samacheer Kalvi for Botany [English] Class 12 TN Board.
Samacheer Kalvi solutions for Botany [English] Class 12 TN Board 4 Principles and Processes of Biotechnology Evaluation [Pages 117 - 118]
Restriction enzymes are
Not always required in genetic engineering
Essential tools in genetic engineering
Nucleases that cleave DNA at specific sites
both b and c
Plasmids are
circular protein molecules
required by bacteria
tiny bacteria
confer resistance to antibiotics
EcoRI cleaves DNA at
AGGGTT
GTATATC
GAATTC
TATAGC
Genetic engineering is
making artificial genes.
hybridization of DNA of one organism to that of the others.
production of alcohol by using micro organisms.
making artificial limbs, diagnostic instruments such as ECG, EEG etc.,
Consider the following statements:
- Recombinant DNA technology is popularly known as genetic engineering is a stream of biotechnology which deals with the manipulation of genetic materials by man invitro
- pBR322 is the first artificial cloning vector developed in 1977 by Boliver and Rodriguez from E.coli plasmid
- Restriction enzymes belong to a class
of enzymes called nucleases. Choose the correct option regarding the above statements
I & II
I & III
II & III
I, II & III
The process of recombinant DNA technology has the following steps
- amplification of the gene
- Insertion of recombinant DNA into the host cells
- Cutting of DNA at a specific location using a restriction enzyme.
- Isolation of genetic material (DNA) Picks out the correct sequence of steps for recombinant DNA technology.
II, III, IV, I
IV, II, III, I
I, II, III, IV
IV, III, I, II
Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzymes?
5' CGTTCG 3' 3' ATCGTA 5'
5' GATATG 3' 3' CTACTA 5'
5' GAATTC 3' 3' CTTAAG 5'
5' CACGTA 3' 3' CTCAGT 5'
PBR 322, BR stands for
Plasmid Bacterial Recombination
Plasmid Bacterial Replication
Plasmid Boliver and Rodriguez
Plasmid Baltimore and Rodriguez
Which of the following one is used as a Biosensors?
Electrophoresis
Bioreactors
Vectors
Electroporation
Match the following:
| Column A | Column A | ||
| 1. | Exonuclease | a. | add or remove phosphate |
| 2. | Endonuclease | b. | binding the DNA fragments |
| 3. | Alkaline Phosphatase | c. | cut the DNA at terminus |
| 4. | Ligase | d. | cut the DNA at middle |
1 - a, 2 - b, 3 - c, 4 - d
1 - c, 2 - d, 3 - b, 4 - a
1 - a, 2 - c, 3 - b, 4 - d
1 - c, 2 - d, 3 - a, 4 - b
In which techniques Ethidium Bromide is used?
Southern Blotting techniques
Western Blotting techniques
Polymerase Chain Reaction
Agrose Gel Electroporosis
Assertion: Agrobacterium tumifaciens is popular in genetic engineering because this bacteriumis associated with the root nodules of all cereals and pulse crops
Reason: A gene incorporated in the bacterial chromosomal genome gets automatically transferred to the cross with which bacterium is associated.
Both assertion and reason are true. But the reason is the correct explanation of assertion.
Both assertion and reason are true. But reason is not correct explanation of assertion.
Assertion is true, but reason is false.
Assertion is false, but reason is true.
Both assertion and reason are false.
Which one of the following is not a correct statement.
Ti plasmid causes the bunchy top disease
Multiple cloning site is known as Polylinker
Non viral method transfection of Nucleic acid in cell
Polylactic acid is a kind of biodegradable and bioactive thermoplastic.
An analysis of chromosomal DNA using the southern hybridisation technique does not use
Electrophoresis
Blotting
Autoradiography
Polymerase Chain Reaction
An antibiotic gene in a vector usually helps in the selection of
Competent cells
Transformed cells
Recombinant cells
None of the above
Some of the characteristics of Bt cotton are
Long fibre and resistant to aphids
Medium yield, long fibre and resistant to beetle pests
high yield and production of toxic protein crystals which kill dipteran pests.
High yield and resistant to ball worms
How do you use the biotechnology in modern practice?
What are the materials used to grow microorganism like Spirulinal
You are working in a biotechnology lab with a becterium namely E.coli. How will you cut the nucleotide sequence? explain it.
What are the enzymes you can use to cut the terminal end and internal phospho di ester bond of nucleotide sequence?
Name the chemicals used in gene transfer.
What do you know about the word pBR332?
Mention the application of Biotechnology.
What is a restriction enzyme? Mention their type with a role in Biotechnology.
Is their any possibilities to transfer a suitable desirable gene to the host plant without a vector? Justify your answer.
How will you identify a vectors?
Compare the various types of Blotting techniques.
Write the advantages of herbicide-tolerant crops.
Write the advantages and disadvantages of Bt cotton.
What is bioremediation? give some examples of bioremediation.
Write the benefits and risk of Genetically Modified Foods.
Solutions for 4: Principles and Processes of Biotechnology
Samacheer Kalvi solutions for Botany [English] Class 12 TN Board chapter 4 - Principles and Processes of Biotechnology
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Concepts covered in Botany [English] Class 12 TN Board chapter 4 Principles and Processes of Biotechnology are Development of Biotechnology, Historical Perspective, Traditional Biotechnology, Advancements in Modern Biotechnology, Tools for Genetic Engineering, Methods of Gene Transfer, Screening for Recombinants, Commercial Applications of Biotechnology, Transgenic Plants / Genetically Modified Crops (Gm Crops).
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