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Question
What is a restriction enzyme? Mention their type with a role in Biotechnology.
Solution
Restriction enzymes are the enzymes of bacterial origin that cleave DNA into fragments at or near specific recognition sites within DNA molecules. This principle is used in biotechnology to cut and insert the desired gene (gene of interest) thereby generating an rDNA with desirable characters.
RELATED QUESTIONS
Restriction enzymes are
Consider the following statements:
- Recombinant DNA technology is popularly known as genetic engineering is a stream of biotechnology which deals with the manipulation of genetic materials by man invitro
- pBR322 is the first artificial cloning vector developed in 1977 by Boliver and Rodriguez from E.coli plasmid
- Restriction enzymes belong to a class
of enzymes called nucleases. Choose the correct option regarding the above statements
Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzymes?
What are the enzymes you can use to cut the terminal end and internal phospho di ester bond of nucleotide sequence?
Name the chemicals used in gene transfer.
How will you identify a vectors?
