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Question
What do you know about the word pBR332?
Solution
PBR 322 plasmid is a reconstructed plasmid and most widely used as a cloning vector; it contains 4361 base pairs. In pBR, p denotes plasmid, B, and R respectively the names of scientist Roliver and/fodriguez who developed this plasmid. The number is the number of plasmid developed from their laboratory.
pBR 322
It contains ample and tetR two different antibiotic resistance genes and recognition sites for several restriction enzymes. (Hind III, EcoRI, BamH I, Sal I, Pvu II, Pst I and Cla I), ori and antibiotic resistance genes. Rop codes for the proteins involved in the replication of the plasmid.
RELATED QUESTIONS
Genetic engineering is
The process of recombinant DNA technology has the following steps
- amplification of the gene
- Insertion of recombinant DNA into the host cells
- Cutting of DNA at a specific location using a restriction enzyme.
- Isolation of genetic material (DNA) Picks out the correct sequence of steps for recombinant DNA technology.
Match the following:
| Column A | Column A | ||
| 1. | Exonuclease | a. | add or remove phosphate |
| 2. | Endonuclease | b. | binding the DNA fragments |
| 3. | Alkaline Phosphatase | c. | cut the DNA at terminus |
| 4. | Ligase | d. | cut the DNA at middle |
Assertion: Agrobacterium tumifaciens is popular in genetic engineering because this bacteriumis associated with the root nodules of all cereals and pulse crops
Reason: A gene incorporated in the bacterial chromosomal genome gets automatically transferred to the cross with which bacterium is associated.
Which one of the following is not a correct statement.
How do you use the biotechnology in modern practice?
