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प्रश्न
Short Answer Question:
Match the entries in column I with those of column II and choose the correct answer.
| Column I | Column II |
| A. Alkali treatment | i. Separation of DNA fragments on gel slab |
| B. Southern blotting | ii. Split DNA fragments into single strands |
| C. Electrophoresis | iii. DNA transferred to nitrocellulose sheet |
| D. PCR | iv. X-ray photography |
| E. Autoradiography | v. Produce fragments of different sizes |
| F. DNA treated with REN | vi. DNA amplification |
उत्तर
| Column I | Column II |
| A. Alkali treatment | ii. Split DNA fragments into single strands |
| B. Southern blotting | iii. DNA transferred to nitrocellulose sheet |
| C. Electrophoresis | i. Separation of DNA fragments on gel slab |
| D. PCR | vi. DNA amplification |
| E. Autoradiography | iv. X-ray photography |
| F. DNA treated with REN | v. Produce fragments of different sizes |
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संबंधित प्रश्न
Differentiate between the following:
Repetitive DNA and Satellite DNA
What is DNA fingerprinting?
Mention how does DNA polymorphism arise in a population.
Long Answer Question:
Guess (i) the possible location of DNA on the collected evidence from a crime scene and (ii) the possible sources of DNA.
| Evidence | Possible location of DNA on the evidence | Sources of DNA |
| e.g. Eyeglasses | e.g. Ear pieces | e.g. Sweat, Skin |
| Bottle, Can, Glass | Sides, mouthpiece | __________ |
| __________ | Handle | Sweat, skin, blood |
| Used cigarette | Cigarette butt | ____________ |
| Bite mark | ____________ | Saliva |
| __________ | Surface area | Hair, semen, sweet, urine |
What is the use of southern blotting in DNA fingerprinting?
Identify the CORRECT description of DNA fingerprinting.
____________ paper is used for embedding DNA strands in Southern blotting technique.
Match the Column - I with Column - II and select the correct option
| Column - I | Column - II | ||
| (a) | Female banded Krait | (i) | Humulin |
| (b) | Pancreas from dog | (ii) | DNA sample |
| (c) | Hair root of human | (iii) | Insulin extracted and purified |
| (d) | E.coli | (iv) | DNA probe |
Select the mis-matched pair.
Arrange the following steps of DNA fingerprinting in correct sequence:
- Gel electrophoresis
- Isolation of DNA
- Southern blotting
- Restriction digestion
