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Why did Hershey and Chase use radioactively labelled phosphorous and sulphur only? Would they have got the same result if they use radiolabelled carbon and nitrogen? - Zoology

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प्रश्न

Why did Hershey and Chase use radioactively labelled phosphorous and sulphur only? Would they have got the same result if they use radiolabelled carbon and nitrogen?

टीपा लिहा

उत्तर

Generally proteins contain sulphur but not phosphorous and nucleic acid (DNA) contains, phosphorous but not sulphur. Hence Hershey - Chase used radioactive isotopes of sulphur (35S) and phosphorus (32P) to keep separate track of viral protein and nucleic acid in culture medium. The expected result cannot be achieved, if radioactive carbon and nitrogen is used, since these molecules are present in both DNA and proteins.

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पाठ 5: Molecular Genetics - Evaluation [पृष्ठ ८६]

APPEARS IN

सामाचीर कलवी Biology (Zoology) [English] Class 12 TN Board
पाठ 5 Molecular Genetics
Evaluation | Q 33. | पृष्ठ ८६
सामाचीर कलवी Biology (Zoology) [English] Class 12 TN Board
पाठ 5 Molecular Genetics
Evaluation | Q 33. | पृष्ठ ८९

संबंधित प्रश्‍न

Answer the following questions based on Hershey and Chases's experiments:

(a) Name the kind of virus they worked with and why?

(b) Why did they use two types of culture media to grow viruses in ? Explain.

(c) What was the need for using a blender and later a centrifuge during their experiments?

(d) State the conclusion drawn by them after the experiments.


Mention one significant difference between Phenotype and Genotype


Define Frameshift mutations


How did Hershey and Chase prove that DNA is the genetic material?


Explain how the biochemical characterisation (nature) of 'Transforming Principle' was determined, which was not defined from Griffth's experiments.


The two strands of DNA are held together by ______.


Nucleotide arrangement in DNA can be seen by ______.


In Streptococcus pneumoniae ______.


The scientists involved in discovery of DNA as chemical basis of heredity were ______.


During infection of E. coli cells by bacteriophage T2, ______.


Which one is a hereditary disease?


The transforming principle of Pneumococcus as found out by Avery, MacLeod, and McCarty was ______.


Which one among the following was the first genetic material?


Which of the following criteria must a molecule fulfill to act as genetic material?

  1. It should not be able to generate its replica.
  2. It should chemically and structurally be stable.
  3. It should not allow slow mutation.
  4. It should be able to express itself in the form of Mendelian Characters.

Transformation was discovered by ______


In Griffith's transforming principle, if heat killed S - strain is injected into mice, then ______.


Name the scientist who proved experimentally that DNA is the genetic material ______.


Recall the experiments done by Frederick Griffith, Avery, MacLeod and McCarty, where DNA was speculated to be the genetic material. If RNA, instead of DNA was the genetic material, would the heat killed strain of Pneumococcus have transformed the R-strain into virulent strain? Explain.


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