Advertisements
Advertisements
प्रश्न
Write steps involved in gene amplification.
थोडक्यात उत्तर
उत्तर
Denaturation: The reaction mixture is heated to a temperature (90–98 oC) to separate two strands of desired DNA. This is called denaturation
Annealing: The mixture is allowed to cool (40–60 oC) that permits pairing of the primer to the complementary sequences in DNA. This step is called annealing
Extension: The temperature (70–75 oC) allows thermostable Taq DNA polymerase to use single-stranded DNA as template and adds nucleotides. This is called primer extension. It takes arround two minutes duration.
shaalaa.com
या प्रश्नात किंवा उत्तरात काही त्रुटी आहे का?
